Evaluation of microvascular dysfunction in pig dilated cardiomyopathy model using a pressure guidewire

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K17080
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Osaka University
• Principal Investigator: Suzuki Kota 大阪大学, 医学部附属病院, 医員 (70816850)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 拡張型心筋症 / iPS細胞 / iPS由来心筋細胞シート

Outline of Final Research Achievements

We performed iPS cell-derived cardiomyocyte sheet transplantation in a pig model of dilated cardiomyopathy and evaluated the effect of the transplantation on cardiac function and the involvement of improvement of coronary microvascular dysfunction as a mechanism of improvement. Cardiac function was significantly improved in the iPS cell sheet transplantation group, but microvascular dysfunction could not be accurately evaluated because of the size of the coronary arteries and the ease with which coronary spasm can be induced. The method using a pressure guidewire was abandoned and myocardial blood flow capacity was evaluated using ammonia PET, which showed significant improvement in myocardial blood flow during maximal hyperemia in the left anterior descending and circumflex regions, suggesting that iPS cell-derived myocardial cell sheets may improve impaired cardiac function. This suggests that iPS cell-derived cardiomyocyte cell sheets may improve impaired cardiac function.

Free Research Field

iPS由来心筋細胞

Academic Significance and Societal Importance of the Research Achievements

拡張型心筋症は心臓移植を必要とする重症心不全の原因として最も多い疾患である。薬物療法を含む内科的治療抵抗性の重症拡張型心筋症に対する治療は、現状では補助人工心臓および心臓移植しかない。しかし、補助人工心臓と心臓移植はそれぞれ耐久性と移植ドナーの少なさという問題を抱えている。拡張型心筋症モデル動物にiPS細胞由来心筋細胞シート移植治療が有効である可能性が示唆されたことは、今後の重症心不全の治療の選択肢を広げる可能性がある。