Development of novel cell administration method to augment tissue regenerative effect for critical limb ischemia

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17150
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Osaka University
• Principal Investigator: Miyake Keisuke 大阪大学, 医学部附属病院, 特任助教(常勤) (60774495)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 下肢虚血

Outline of Final Research Achievements

Regenerative therapy has been expected to be a new solution for some ischemic disease that are difficult to treat with current medicine. However, in such ischemic diseases, severe ischemic and inflammatory environment of the disease make administered regenerative agents difficult to survive enough to show regenerative effect. Therefore to develop new and effective regenerative therapy, to solve the low survival rate of administered cells in the harsh environment is a prerequisite.
In this study, we developed a way to improve the cell survival rate in the harsh ischemic and inflammatory environment: clustered cells that are composed of cells connected with extracellular matrices enabled to maximize cell survival rate and therefore showed high regenerative capacity in the severe ischemic status of lower limbs.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

本研究では、細胞再生医療を虚血領域に直接投与する場合には、細胞単体投与はほとんど効果がなく、投与方法を改良し、細胞生着率を高める方法を用いなけれ ば、十分な治療効果をもたらすことが難しいが、生着率を高める方法を用いれば、再生治療効果をもたらしうることが明らかとなった。本研究は、実臨床での応 用が期待される手法であり、今後、臨床試験に向けた手続きを取り組んでいく予定である。