Contribution of the miRNAs derived from EBV for mosquito bite hypersensitivity

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17362
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Tokai University
• Principal Investigator: Miyatake Yuji 東海大学, 医学部, 特任助教 (30868881)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 蚊刺過敏症 / BART / EBV

Outline of Final Research Achievements

To reveal the relation between RNA region BART which is unique in EB virus and mosquito bite hypersensitivity, we generated the mouse systemically expressing BART and found out the mouse showed severe skin itching and elevated blood IgE density. Next, though we generated blood lineage specific BART expressing mouse by Cre-loxP system, the mouse did not show conspicuous phenotypes. Also a single injection of mosquito salivary extracts did not cause intense immune reactions to these BART expressing mice.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

EBウイルス由来因子の中でも小分子RNA群のBARTが免疫反応やアレルギー様症状に対して一定の役割を担っていることを示した本研究は、発症機序が未知であるEBウイルス関連疾患の理解を深めるものである。また、本研究で作出されたBARTを組織特異的に発現するマウスは今後のEBウイルス関連疾患の分子機序を解明するうえで非常に重要なものである。