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2023 Fiscal Year Final Research Report

Clarification of pathogenicity of Difficult to treat rheumatoid arthritis using by multi-omics analysys of synovial tissue

Research Project

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Project/Area Number 20K17450
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 54020:Connective tissue disease and allergy-related
Research InstitutionKeio University

Principal Investigator

Kondo Yasushi  慶應義塾大学, 医学部(信濃町), 助教 (50626380)

Project Period (FY) 2020-04-01 – 2024-03-31
Keywords滑膜 / 滑膜生検 / マスサイトメトリー
Outline of Final Research Achievements

We recruited a total of 18 patients with arthritis including, 6 patients with difficult to treat rheumatoid arthritis (D2T-RA), 3 patients with typical RA (defined as untreated, anti-CCP antibody-positive) and other arthritis as a control group. In addition to general histopathological and immunohistological analysis, detailed cell subset analysis by mass cytometry using the CyTOF system and comprehensive analysis of gene expression by single-cell RNA sequencing were performed, although the analysis is still in progress at this point. We identified that specific subsets of synovial infiltrating cells obtained from D2T-RA and elderly-onset rheumatoid arthritis.

Translated with DeepL.com (free version)

Free Research Field

リウマチ・膠原病

Academic Significance and Societal Importance of the Research Achievements

本研究では超音波ガイド下低滑膜生検法を用いることで、ヒト関節リウマチの病変滑膜を直接採取することが特徴の1つであった。採取した滑膜組織は、手術による生検組織に比べて、十分な組織量が採取できないことが問題点であったが、実際に今回得られた滑膜からフローサイトメトリー、CyTOFシステムを用いたマスサイトメトリーや、単一細胞(シングルセル)解析を行い、疾患特異的な新規細胞集団と考えられる分画が見いだせた点が本研究の学術的意義であったと考えられる。今後、治療抵抗性RAに対する薬剤反応性や副作用などの予後予測への応用が期待され、個別化医療の実現に繋がる端緒となりうることが社会的意義と考える。

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Published: 2025-01-30  

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