2021 Fiscal Year Final Research Report
Elucidation of the mechanism by which the colorectal cancer microenvironment is involved in carcinogenesis using scRNAseq and gut microbiota analysis
| Project/Area Number |
20K17621
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55020:Digestive surgery-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | scRNAseq / 大腸がん / 腸内細菌叢 / 微小環境 |
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| Outline of Final Research Achievements |
Fecal samples were collected from patients with colorectal cancer, and a database was created. At the same time, we collected samples from gastrointestinal cancers, including colorectal cancer, and processed them for single cell analysis. The data after NGS analysis were analyzed using the R package Seurat, focusing on the characteristics and functions of the cell population from the expression of RNA derived from single cells, and the analysis of pseudo genealogical data to determine the direction of differentiation of the cells. We have not yet been able to compare the data with the results of bacterial flora analysis, PDX mouse model, or establishment of colon cancer organoids.
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| Free Research Field |
医歯薬学
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| Academic Significance and Societal Importance of the Research Achievements |
大腸癌の発癌メカニズム及び治療抵抗性を腸内細菌叢及び腫瘍微小環境の不均一性という側面から検討することは、大腸癌治療開発において全く新たな切り口でもあり、罹患者及び死亡者の数の多さから特に社会的な要請の強い大腸癌の治療開発に飛躍的な進歩をもたらすと期待される。また、本成果は、scRNAseq解析やMicrobiome解析などNGS解析の膨大なデータを含むもので、そのインパクトは大きく、癌研究だけでなく、生物学や細菌学など学術的にも広範な波及効果が期待される。
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