2021 Fiscal Year Final Research Report
Functional elucidation of SWI/SNF complex in colorectal cancer
Project/Area Number |
20K17684
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 55020:Digestive surgery-related
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Research Institution | Osaka University |
Principal Investigator |
Miyo Masaaki 大阪大学, 医学系研究科, 招へい教員 (70645077)
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Project Period (FY) |
2020-04-01 – 2022-03-31
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Keywords | SWI/SNF複合体 / 大腸癌 |
Outline of Final Research Achievements |
The public database analysis revealed that the subunit of SWI/SNF complex such as SMARCA4, SMARCC1 co-localized with a certain cancer-associated transcription factor (TF) in promoter and enhancer regions of several cancer-related genes. Therefore, we performed co-immunoprecipitation experiments using antibodies against the TF and the subunit of SWI/SNF complex (SMARCA4, C1, C2, ARID1A). As a result, the precipitants by the antibody against the subunit of SWI/SNF complex (SMARCA4, C1, C2, ARID1A) contained this TF. On the other hand, the precipitants by the antibody raised against the TF somehow did not contain the subunit of SWI/SNF complex (SMARCA4, C1, C2, ARID1A). This might be because the TF makes complex with so many molecules. Therefore, although further investigation is required, our results suggest the possibility that this cancer-associated TF may be a new subunit of SWI/SNF complex.
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Free Research Field |
消化器外科
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Academic Significance and Societal Importance of the Research Achievements |
SWI/SNF複合体は多数の腫瘍でそのサブユニットの遺伝子変化が報告され、癌の発生や進行に深く関与していることが示唆されている。しかしながら、癌制御のメカニズムについては依然として不明な点が多く、未知のサブユニットの存在も示唆されている。本研究ではSWI/SNF複合体の新たなサブユニット候補として癌関連転写因子を同定することに成功しており、新たなSWI/SNF複合体のサブユニットを標的とした新規の癌マーカーや治療法の開発に繋がることが期待される。
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