Comprehensive elucidation of CBLC expression in gastrointestinal cancer progression

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K17700
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Nara Medical University
• Principal Investigator: Miyao Shintaro 奈良県立医科大学, 医学部附属病院, 研究員 (00833708)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 食道がん / ユビキチン

Outline of Final Research Achievements

The purpose of this study is to comprehensively elucidate the mechanism of cancer progression mediated by ubiquitin modification system abnormalities in gastrointestinal cancers such as gastric cancer and esophageal cancer. First, esophageal cancer cell lines (TE-6, TE- When the gene expression in 8) was confirmed, it was confirmed that the heterogeneity was very large even among the cell lines. Therefore, the mechanism elucidated based on the gene expression analysis derived from a single cell from the originally planned analysis in bulk. It was confirmed that nucleic acid solubilization becomes possible and cDNA synthesis becomes possible by introducing dissociation of methylene cross-linking by proteolysis and heat treatment into the existing single cell RNA-seq process.

Free Research Field

上部消化器癌

Academic Significance and Societal Importance of the Research Achievements

当初の目的とは異なる結果となったが, FFPE標本からの微量解析が可能となる兆候が発見されたことは非常に社会的意義のあることと考える. 本手法を本格的に検討することで, あらゆる疾患分野で画期的なブレイクスルーが得られる可能性や、従来の固定標本からの検査の精度をさらに上昇させることも可能と考える.