The research for the relation between an abnormal increase in circuit pressure and leukocyte-derived extracellular DNA in cases using cardiopulmonary bypass

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17705
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Yamagata University
• Principal Investigator: Yashima Nozomi 山形大学, 医学部, 助教 (70637339)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 人工心肺 / 回上昇上昇 / 白血球 / 細胞外DNA

Outline of Final Research Achievements

When using a cardiopulmonary bypass (CPB), clots can form and cause an abnormal increase in circuit pressure, but the mechanism has not been cleared. Through experiment using a simulated circuit, we concluded that the cause of the increase in circuit pressure was extracellular DNA derived from leukocytes. In this study, we investigated the relation between the circuit pressure of CPB and leukocytes, extracellular DNA concentration, and coagulation system marker values in cardiac surgery using the CPB.

Free Research Field

血栓止血学

Academic Significance and Societal Importance of the Research Achievements

人工心肺は患者の生命を維持するために必須の装置であり、そのトラブルは患者の生死にかかわることである。これまでに人工心肺の回路内凝血・回路圧上昇に関して白血球由来細胞外DNAが関与しているとする研究はほとんどない。本研究の検証で回路内凝血・回路圧上昇に白血球由来細胞外DNAが関与していることが明らかになれば、回路内凝血・回路圧上昇のメカニズムが解明され、その対処法・予防法を確立することができる可能性がある。