Role of NLRP3 in cardiac allograft rejection

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17711
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Shinshu University
• Principal Investigator: GOMIBUCHI Toshihito 信州大学, 医学部, 特任助教 (90597668)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 心移植 / NLRP3

Outline of Final Research Achievements

We used a murine heterotopic cardiac transplantation model using NLRP3 knockout mice. There is no difference in graft survival of fully mismatched model compared to control group. Donor hearts were harvested for examination on Days 5. In NLRP3 knockout group, a severe infiltration of inflammatory cells was observed in the graft and more myocardial injury was seen. The expression of Caspase-3、ICAM1、CD45, CD3, CD31, αSMA, NLRP3 was analyzed by immunohistochemistry.

Free Research Field

心臓血管外科

Academic Significance and Societal Importance of the Research Achievements

これまでに、NLRP3と移植免疫についての研究は無く、これまでにない独創的な計画であると考えられる。DNA修復酵素であるNLRP3が移植後の拒絶反応を抑える新たな標的分子となる可能性が示唆された。