Investigation of calcium sensitivity and protein relationship between cMyBP-C and Fhod3 in failing human myocardium.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17721
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Kyushu University
• Principal Investigator: matsuyama sho 九州大学, 医学研究院, 共同研究員 (00857782)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 心不全 / 不全心筋 / Fhod3 / cMyBP-C蛋白質

Outline of Final Research Achievements

In an initial experiment, skinned fibers were generated in a rabbit model simulating failing myocardium. Subsequently, the contractility and calcium sensitivity of these fibers were evaluated employing myosin inhibitors, actin polymerization inhibitors, and phosphorylating agents, while making use of equipment loaned from external institutions. The reproducibility of the outcomes was confirmed. The anticipated number of human myocardial samples was estimated, and an application was being prepared for submission to the Ethics Review Committee for Clinical Research at this hospital. However, tangible progress was impeded by the reassignment of the principal investigator, thereby preventing the elucidation of the mechanism employing human myocardial samples.

Free Research Field

心不全

Academic Significance and Societal Importance of the Research Achievements

前実験としてウサギでの不全心筋モデルにてskinned fiberを作成し、他施設の設備を借用し、ミオシン阻害剤、アクチン重合阻害剤、リン酸化剤を使用した際の収縮力・Ca感受性を調べ、その再現性を確認する手技を行っていたが、ヒト心筋サンプルでのメカニズム解明に関しては研究が思うように進まず、今回の研究成果の意義としては、満足のいくものではなかった。