Examination of relation between nuclear atypia and cytomorphological features for lung adenocarcinoma sub-types based on driver mutation

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17738
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55040:Respiratory surgery-related
• Research Institution: Gunma University
• Principal Investigator: Sayaka Kobayashi 群馬大学, 大学院保健学研究科, 助教 (80765694)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 肺腺癌 / 遺伝子変異特異的microRNA / EGFR遺伝子変異 / KRAS遺伝子変異 / エメリン / ラミン / 核の形状因子 / 臨床病理学的所見

Outline of Final Research Achievements

We revealed that driver mutation-specific microRNA did not change specifically even if lung adenocarcinoma cells have EGFR or KRAS mutation. These results were published in Japanese.
In addition, we examined the association between expression of emerin, lamin A, B1, B2 and nuclear morphological factor (nuclear area, perimeter and shape factor), clinicopathological features in human lung adenocarcinoma cases utilizing formalin-fixed paraffin-embedded tissues by immunohistochemistry. These results are summarized and being submitted to a journal.

Free Research Field

病理組織・細胞診断学

Academic Significance and Societal Importance of the Research Achievements

ヒト組織に対する遺伝子変異特異的microRNA解析は他の手法よりも比較的簡便な手法であるが、上記microRNAは培養細胞の検討で癌細胞そのもののEGFR，KRAS変異を反映せず、EGFR，KRA変異検査としては使用できないことが明らかとなり、臨床的に意義深い。
またエメリン，ラミンの発現の有無と核所見や臨床病理学的所見（特に組織亜型）との関連を明らかにしたことは我々が初めてであり学術的意義がある。