2023 Fiscal Year Final Research Report
Examination of the effect of oxytocin on non-nociceptive and nociceptive stimuli in the upper central nervous system and the mitigation factor
| Project/Area Number |
20K17773
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55050:Anesthesiology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | オキシトシン / 疼痛制御 / 術後痛 |
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| Outline of Final Research Achievements |
Behavioral experiments were conducted using wild-type mice (WT), oxytocin knockout mice (Oxt-/-), receptor knockout mice (Oxtr-/-) and mice OxtrVenus/+ in which the fluorescent protein Venus was knocked in at the receptor knockout site. As a result of observing the effects of OXT on responses to nociceptive and non-nociceptive stimuli, a significant difference was observed between the Hargreaves test and the von-Frey test.
Furthermore, we examined colocalization with oxytocin receptors using an antibody against (c-fos), a neural activation marker, on brain slices prepared after the Hargreaves test and the von-Frey test, in which a significant difference was found. We showed that oxytocin exerts analgesic effects via the aqueductal gray matter and effects on emotion via the medial amygdala nucleus and nucleus accumbens.
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| Free Research Field |
麻酔科学
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| Academic Significance and Societal Importance of the Research Achievements |
侵害刺激および痛み刺激に対するオキシトシンおよびオキシトシン受容体の局在ならびに役割、さらには、関連するイオンチャネルもしくは受容体を明らかにすることで、それぞれの反応に対する鎮痛作用もしくは抗ストレス作用を発揮する薬物候補を検索することが可能となる。加えて、脳内のオキシトシン受容体を介した疼痛制御のメカニズムを解明し、本結果を行動学的試験にフィードバックすることで、臨床応用の可能性を探る。
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