Establishment of an ultra-compact sequencing system that enables rapid on-site diagnosis of infectious diseases at emergency sites

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17880
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Kansai Medical University
• Principal Investigator: NAKAMURA Fumiko 関西医科大学, 医学部, 助教 (80701721)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: MinION / 16SリボソームRNA遺伝子 / 腹水 / 腹腔内感染症

Outline of Final Research Achievements

The study involved 16 patients with generalized peritonitis who were rushed to our hospital and underwent emergency abdominal surgery. Ascites was collected during surgery and the resulting samples were divided. Even in samples where the results of general bacterial culture were negative, it was possible to identify bacteria using the MinION. The rapid bacterial identification method using the MinION enabled highly accurate discrimination and identification of bacteria, making it a useful tool for diagnosing infectious diseases.

Free Research Field

腹部救急

Academic Significance and Societal Importance of the Research Achievements

今回、16SrRNA遺伝子を指標としたMinIONを用いた迅速細菌同定法の有用性を検証し、臨床的アウトカムの向上に繋がる技術基盤を確立することを目的とした。当院に救急搬送され、腹部緊急手術を行った汎発性腹膜炎患者を対象とした。
MinIONによる迅速細菌同定法はより高精度に細菌の判別・同定を行うことができ、感染症ツールとして有用である。