2021 Fiscal Year Final Research Report
A novel nerve reconstruction technique using liquid nitrogen-treated tumor-bearing nerve graft
| Project/Area Number |
20K18025
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56020:Orthopedics-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | 骨軟部腫瘍 / 末梢神経 / 自家神経移植 |
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| Outline of Final Research Achievements |
Although the liquid nitrogen treatment has been used for malignant bone tumor sterilization, its use in the preparation of nerve grafts has hitherto not been reported. Despite tumor involvement and freezing treatment, tumor-bearing frozen grafts demonstrated nerve regeneration activity, with no local recurrence observed at 48 weeks postoperatively in nude rats in this study. Our study revealed that the frozen nerve autograft has a similar regenerative potential as that of a nerve autograft. Though the findings in a rat model do not guarantee efficacy in humans, if they are substantiated by large-animal models, clinical trials will be needed for evaluating the efficacy of the tumor-bearing frozen nerve grafts in humans.
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| Free Research Field |
整形外科
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| Academic Significance and Societal Importance of the Research Achievements |
筋肉や神経，脂肪組織に発生した軟部肉腫の標準的な治療方法は手術であり，しかも軟部肉腫を正常組織で包み込むようにして切除（広範切除）することが必要となる．広範切除を行うにあたり，十分な切除縁を得るためには，ときに神経も合併切除する必要がある．本実験は，担癌神経グラフトを液体窒素処理し再利用する手術方法を考案した．この方法がヒトに応用できるようになれば，自家神経移植のように採取部の障害がなく，さらに切除した長さと等長の神経グラフトを得ることができ，軟部肉腫広範切除後の患肢再建の一助となることが予想される．
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