Thr effects of platelet-rich fibrin on Achilles tendon injury and tenocytes

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18029
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Mie University
• Principal Investigator: Senga Yoshiyuki 三重大学, 医学部附属病院, 助教 (70828368)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 多血小板フィブリン / PRF / アキレス腱 / 再生医療 / 成長因子 / スポーツ外傷・障害 / シグナル

Outline of Final Research Achievements

Platelet Rich Fibrin (PRF) is a fibrin mass containing high growth factors concentrations. It is expected to promote healing in various pathological conditions due to its sustained release properties. We evaluated PRF directly on Achilles tendon defects in an animal model. We aimed to identify its effect on tenocytes, which play an essential role in tendon repair, and the signaling pathways involved. We found that PRF promotes tenocyte proliferation via FGF receptor/Akt signaling and tenocyte activation via TGF-β receptor/Smad3 signaling to promote Achilles tendon injury healing.

Free Research Field

整形外科/スポーツ医学/膝・足の外科/再生医療

Academic Significance and Societal Importance of the Research Achievements

本研究を通じて、PRFはアキレス腱損傷の治癒促進に有用であること、特にPRFに含まれるFGF受容体/Aktシグナルを介してtenocyteの増殖を促し、 TGF-β受容体/Smad3シグナルを介してtenocyteを活性化させるというアキレス腱損傷治癒のメカニズムを明らかにした。本研究によりPRFを直接アキレス腱損傷の治療に応用できる可能性や特に重要な成長因子であるFGFやTGF-βを腱損傷の治療に応用できる可能性が示唆されており、今後の新規治療法の開発への一助になったものと考える。