Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18057
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Kanazawa University
• Principal Investigator: Yoshitani Junya 金沢大学, 医学系, 協力研究員 (30800601)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 脂肪由来幹細胞 / インプラント周囲感染症 / 骨髄炎

Outline of Final Research Achievements

This study aimed to evaluate the antimicrobial activity and therapeutic effect of local treatment with antibiotic-loaded adipose-derived stem cells (ADSCs) plus an antibiotic in a rat implant-associated infection model. Liquid chromatography/tandem mass spectrometry revealed that ADSCs cultured in the presence of ciprofloxacin for 24 h showed time-dependent antibiotic loading. The therapeutic effects of ADSCs plus antibiotics, antibiotics, and ADSCs were compared with no treatment as a control. Rats treated with ADSCs plus ciprofloxacin had the lowest modified osteomyelitis scores, abscess formation, and bacterial burden on the implant among all groups (P < 0.05). Thus, local treatment with ADSCs plus an antibiotic has an antimicrobial effect in implant-related infection and decrease abscess formation. Thus, our findings indicate that local administration of ADSCs with antibiotics represents a novel treatment strategy for implant-associated osteomyelitis.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

脂肪由来幹細胞を用いた新たな感染治療の開発を行った。これまで難治性であったインプラント周囲感染症において、抗生剤と脂肪由来幹細胞を併用することで従来の治療を上回る効果が得られた。この結果はScientific Reports誌に投稿しまた海外でも発表を行っており一定の評価を得ている。今後は臨床試験へ向けて、大動物での実験系統を開発し、脂肪由来幹細胞と抗生剤の合剤として製薬開発を考慮する。本研究は新たなインプラント周囲感染症治療の基礎となるものである。