Novel bone regeneration therapy using Rspo2

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18059
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Nagoya University
• Principal Investigator: NAKASHIMA HIROAKI 名古屋大学, 医学系研究科, 准教授 (70710101)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 骨再生 / Wnt / Rspo2

Outline of Final Research Achievements

The molecular mechanisms underlying the reciprocal signalling induced by BMP2 and Rspo2 in osteoblast osteogenesis were elucidated. Together with the promotion of bone formation in lumbar interbody fusion using self-assembling peptides as scaffolds, which has been studied in our department, we have confirmed that these self-assembling peptides containing BMP2 and Rspo2 rapidly induce endogenous osteoblasts to scaffolds and promote bone formation in experiments. In order to develop a new treatment for pseudoarthrosis, the concentrations of BMP2 and Rspo2 suitable for osteogenic differentiation were determined. By combining self-assembling peptides, the minimum required concentration could be reduced and the 'inflammation and other side effects' previously observed with the dissemination of the secreted factor BMP2 could be suppressed.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

高齢者の増加に伴い骨粗鬆症が増加している。高齢者では骨再生が不完全となるため、骨折後の偽関節のみならず、脊椎やその他の整形外科領域の矯正固定術後に偽関節となる症例も多く認めるため、速やかな骨癒合を促進する因子が求められてきた。この偽関節予防・治療に対しBMP2などの分泌される骨誘導因子を用いた骨再生療法の臨床治験が諸外国では始まったが、実臨床では期待された骨再生効果が得られず、周囲組織の炎症などの副作用も多いため本邦では認可されていない。本研究での自己組織化ペプチドとBMP2、Rspo2の混合製剤の使用により、安全で効果的な骨再生が期待できる。