Roles of mitochondrial function in temperature intervention during recovery from muscle fatigue

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18069
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Sapporo Medical University
• Principal Investigator: Fusagawa Hiroyori 札幌医科大学, 医学部, 助教 (30868112)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: ミトコンドリア / 骨格筋 / 温度介入

Outline of Final Research Achievements

Evidence for thermal intervention in post-exercise muscle fatigue is still lacking. Wistar rats were subjected to thermal intervention for 30 minutes after 30 minutes of treadmill running at a speed of 10-20 m/min. The lower legs were exposed to room temperature or a 42 degree hot water tank or a 16 degree cold water tank. Signal analysis of fusion, fission and autophagy, which are responsible for quality control of mitochondria, was performed on collected muscle samples. It was confirmed that the cooling intervention maintained the phosphorylation level of DRP1-Ser616 at a high level.

Free Research Field

骨格筋生理学

Academic Significance and Societal Importance of the Research Achievements

運動直後にはアイシング、冷却が行われているがエネルギー会社の観点からはむしろ温熱の方が有効であるという報告も多い。今回冷却介入を行うことでミトコンドリアダイナミクスに影響を与えうるfissionシグナルが高く維持されていた。これは運動後冷却介入を繰り返すことでミトコンドリアを過度に分裂させミトコンドリアの形態変化を伴うエネルギー代謝に影響を与える可能性を示唆した。単回の温度介入では温熱、冷却ともに筋疲労回復に影響を与えないが、繰り返し運動後に介入することでミトコンドリア形態変化、疲労耐性に影響を与えることが示唆された。