The Role and Interaction of TRPA1 Cation Channels and Nitric Oxide in Skin Wound Healing

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18071
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Wakayama Medical University
• Principal Investigator: Murata Shizumasa 和歌山県立医科大学, 医学部, 博士研究員 (90838294)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 創傷治癒

Outline of Final Research Achievements

This study examined if the loss of transient receptor potential ankyrin 1 (TRPA1) affects skin wound healing in mice. We evaluated granulation tissue formation by myofibroblasts and macrophages, re-epithelialization, and related gene expression. Using TRPA1-null (KO) and wild-type (WT) C57BL/6 mice, we created two full-thickness excision wounds (5.0 mm diameter) on their dorsal skin. Healing was assessed via macroscopic observation, histology, immunohistochemistry, and RT-PCR at specific intervals. TRPA1 KO delayed granulation tissue formation and re-epithelialization, suppressed myofibroblast appearance, macrophage infiltration, and mRNA expression of αSMA, F4/80, and Col-1α2. These results indicate that TRPA1 is crucial for skin wound healing in mice, as its absence delays macrophage infiltration and subsequent fibrotic tissue formation, impairing fibroblast fibrogenic behavior.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

この研究は、TRPA1カチオンチャネルが皮膚創傷治癒において重要な役割を果たすことを示しています。TRPA1の欠損が肉芽組織形成や再上皮化を遅延させ、マクロファージの浸潤や線維芽細胞の機能に影響を与えることが明らかになりました。これにより、創傷治癒におけるTRPA1の新たな治療標的としての可能性が示唆されます。社会的には、創傷治癒を促進する新しい治療法の開発につながり、特に糖尿病や慢性創傷を持つ患者に大きな利益をもたらす可能性があります。