2022 Fiscal Year Final Research Report
A novel hybrid nerve conduit for peripheral nerve regeneration
Project/Area Number |
20K18074
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56020:Orthopedics-related
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Research Institution | Keio University |
Principal Investigator |
Kimura Hiroo 慶應義塾大学, 医学部(信濃町), 助教 (40627048)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 末梢神経再生 / 人工神経 / 神経損傷 / 再生医療 / 神経麻痺 |
Outline of Final Research Achievements |
We prepared a chitosan-collagen hydrogel nerve conduit (CCN) consisting of an outer layer of chitosan hydrogel and an inner layer of collagen hydrogel to encapsulate the intended cells. Rats with a 10-mm sciatic nerve defect were treated with Schwann cells encapsulated in CCN (CCN+), CCN without SCs (CCN-), SC-encapsulated silicone tube (silicone+), and autologous nerve transplanting (auto). Behavioral and histological analyses indicated that motor function recovery and myelination of the CCN+ group were superior to those of the CCN- and silicone+ groups, whereas the CCN- and silicone+ groups showed no significant differences in the recovery of motor function and nerve restoration. In conclusion, SC-encapsulated CCN possessed synergistic effect on peripheral nerve regeneration, especially remyelination of host SCs. The comparable recovery in the CCN- and silicone+ groups suggests that the structure of CCN and/or the effect of chitosan metabolites positively impact nerve regeneration.
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Free Research Field |
末梢神経障害
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Academic Significance and Societal Importance of the Research Achievements |
細胞移植を前提にした生体吸収性の人工神経を作成してラット末梢神経欠損部に移植し、神経再生が促進されたことを示した。現在、細胞移植を前提にした人工神経は存在しないため、本人工神経が臨床応用可能になれば、既存の人工神経よりも良好な神経再生とそれによる機能回復が得られる可能性が期待される。また、現時点での標準治療である自家神経移植術を回避することができれば、神経採取に伴う様々な問題の解決に繋がる。
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