2022 Fiscal Year Annual Research Report
A study of newly-identified testis-specific long non-coding RNAs in mouse spermatogenesis
| Project/Area Number |
20K18103
|
|---|
| Research Institution | International University of Health and Welfare |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2020-04-01 – 2023-03-31
|
| Keywords | long non-coding RNA / mouse testis / spermatogenesis / testis development / testis-specific lncRNAs |
|---|
| Outline of Annual Research Achievements |
In the previous years, we had been newly identified testis-exclusive lncRNAs in adult mice. In this year, I analyzed the expression patterns of lncRNAs during mouse testicular developmental periods using in silico database. As results, there were similar gene expression patterns in the embryonic and postnatal testis, particularly Malat1 lncRNA expression was the highest in first wave of spermatogenesis.
Environmental contaminants impact on reproduction and developmental toxicity. I observed that a testis-specific lncRNA expression was upregulated after arsenic exposure to spermatocytes in vitro. Genome-wide analysis by RNA-seq revealed that the upregulation of genes was related to spermatogenesis pathway, positive regulation of germ cell proliferation and regulation of apoptosis process.
|
