Clinical significance and related-mechanisms of TRIM44 in bladder cancer

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18134
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56030:Urology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Yamada Yuta 東京大学, 医学部附属病院, 講師 (10376452)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 膀胱癌 / trim44

Outline of Final Research Achievements

Tripartite motif 44 is a previously known oncogenic signal that is associated in head/neck cancer, gastric cancer, pancreatic cancer, breast cancer, colon cancer, cervival cancer, prostate cancer, kidney cancer, and more. We identified Tripartite motif protein (TRIM) 44 as a prognostic factor of bladder cancer based on the immunohistochemical studies. We used bladder cancer cell lines and performed gain-of-function and loss-of-function studies to investigate the function of TRIM44. We performed MTS assay to investigate the relationship of TRIM44 and cell proliferation and found that TRIM44 facilitated tumor growth of bladder cancer cells both in gain-of-function and loss-of-function studies. Migration assay was also performed and TRIM44 promoted migration in bladder cancer cells.

Free Research Field

泌尿器癌

Academic Significance and Societal Importance of the Research Achievements

TRIM44はもともと頭頚部癌、胃癌、大腸癌、乳癌、膵癌、子宮頸がん、肺がん、前立腺癌、腎癌など10種類以上の癌種で癌細胞の増殖を促進する方向に働き癌遺伝子と考えられている。今回、膀胱癌においても免疫学的染色や膀胱癌細胞株を用いた機能実験の結果からTRIM44は癌を促進する作用があることがわかった。TRIM44を治療標的とした化合物が同的できれば治療ターゲットとなり得ることが分かり膀胱癌に対する新規治療薬となる可能性が考えられた。また、TRIM44蛋白は今回血中で同定することができなかった、今後、血清から同定できる手法が確立されれば膀胱癌の病勢を反映するバイオマーカーとなる可能性がある。