Genomic stability studies with early embryonic factor Zscan5b

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K18200
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Saitama Medical University
• Principal Investigator: Nakamura Akihiro 埼玉医科大学, 医学部, 助教 (50750973)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: iPS細胞 / ゲノム安定化

Outline of Final Research Achievements

Metabolome analysis of iPS cells established from 8-week-old, 12-week-old, and 24-week-old mice was performed.Based on previous experiments and reports in papers, iPS cells established from aged mice were expected to have reduced mitochondrial activity and improved by Zscan5b gene transfer. However, there was a large difference between lines of iPS cells derived from the same age, and it was not possible to grasp the tendency of each age. It is future task to clarify what the differences in characteristics between strains are derived from.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では人工多能性幹（iPS: induced Pluripotent stem）細胞を始めとした培養細胞に対し、「初期胚型修復機構」を誘導、再現させることでゲノム安定性の向上を目指した。人工多能性幹(iPS)細胞の老化に伴うゲノム損傷の蓄積や、ゲノム安定性の低下が指摘されており、臨床応用をはじめ、胚性幹(ES)細胞や全能性を有する受精卵と同様の研究素材とするうえでの課題となっている。このような課題に対し本研究ではゲノム修復因子を外部から遺伝子工学的技術を用いて人工的に導入することにより克服することができるのではないかと考え、本研究課題に着手した。