Comprehensive analysis of changes in retinal pigment epithelium-derived lipid mediators by subthreshold micropulse laser

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K18377
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56060:Ophthalmology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Araki Fumiyuki 東京大学, 医学部附属病院, 助教 (20724652)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 糖尿病黄斑浮腫 / 網膜閾値下凝固

Outline of Final Research Achievements

Human iPS cell-derived RPE (retinal pigment epithelium) cells were cultured and blackened, and the laser conditions under which there was no RPE cell death and sufficient HSP70 expression was determined. Supernatants 3 and 24 hours after laser irradiation were collected, and comprehensive lipid analysis of lipid mediators was performed. Autotaxin (ATX) concentration was lower than that of control.

Free Research Field

糖尿病網膜症

Academic Significance and Societal Importance of the Research Achievements

実験用の網膜凝固装置を用いて培養網膜色素上皮細胞に閾値下凝固を行うレーザーの条件を確立し、糖尿病黄斑浮腫に対する網膜閾値下凝固の基礎実験を可能とした。また、細胞死を引き起こさない低侵襲照射において、コントロール群と比較してリゾフォスファチジン酸（LPA）産生酵素であるオートタキシン（ATX）の濃度が低下していることを発見し、脂質メディエーターが網膜に対する低侵襲照射治療に関わっている可能性が示唆された。