2022 Fiscal Year Annual Research Report
iPS細胞由来網膜移植にて視機能再建のための変性網膜の視覚回路の解析と最適化
| Project/Area Number |
20K18403
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| Research Institution | Osaka University |
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Principal Investigator |
Tu HungYa 大阪大学, 蛋白質研究所, 助教 (10780835)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | retinal degeneration / horizontal cell / organoid transplantation / multielectrode array |
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| Outline of Annual Research Achievements |
To clarify the role of horizontal cells (HCs) in photoreceptor synapse reformation after retinal organoid transplantation, tamoxifen-induced conditional HC labeling and ablation model has been established in retinal degeneration mouse background. Mouse retinal organoids were transplanted to the HC-ablated degenerating retinas, and multielectrode array recording was conducted to assess the host-graft reconstruction. The host ganglion cells showed increased light responses with better signal-to-noise ratio compared to the regular transplantation preparation without horizontal cell disturbance. De novo synapses between host bipolar cells and graft photoreceptors were observed with the graft horizontal cell processes invaginated in the horizontal cell ablated retinas. These results indicate that the removal of horizontal cells from the degenerated retinas prior to transplantation facilitates the functional synapse formation between host bipolar cells and graft photoreceptors. The residual horizontal cells may be incapable of forming new synapses and hence physically block the interaction of host bipolar cells and graft photoreceptors upon transplantation.
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