TRP channels involvement in the pathogenesis of intractable lymphedema

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18415
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Wakayama Medical University
• Principal Investigator: Ueno Kazuki 和歌山県立医科大学, 医学部, 助教 (30817028)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: TRPV1 / TRPイオンチャネル / 創傷治癒 / リンパ浮腫 / 好中球 / NETｓ

Outline of Final Research Achievements

We analyzed the role of TRPV1, one of the TRP channels involved in various biological functions, in murine cutaneous wound healing. The results showed delayed wound healing and abnormal re-epithelialization in KO mice. In addition, we observed increased NETs formation and prolonged neutrophilic inflammation. These results suggest that TRPV1 channel may be an important TRP channel in murine cutaneous wound healing that regulates neutrophilic inflammatory responses. We have also succeeded in generating a mouse model of chronic lymphedema of the tail and expect to analyze the involvement of signaling pathways including TRPs by modifying this system.

Free Research Field

創傷治癒、リンパ浮腫、顔面先天異常

Academic Significance and Societal Importance of the Research Achievements

多様な生体機能に関わるTRPチャネルの一つ、TRPV1のマウス皮膚の創傷治癒における役割を解析し、TRPV1チャネルは、好中球性の炎症反応を制御する、マウス皮膚創傷治癒に重要なTRPチャネルである可能性が示唆された。また、マウス尾部慢性リンパ浮腫モデルの作成に成功し、この系を改良する事でTRPを含むシグナル系の関与についても今後解析が期待される。今回の知見を元に、TRPチャネル解析を通じて形成外科領域に重要な創傷治癒とリンパ浮腫双方についての解析進展が期待される。