innovative rejuvenation strategy by supermicrosurgical parabiosis

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K18422
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Keio University
• Principal Investigator: SUZUKI Yushi 慶應義塾大学, 医学部(信濃町), 特任助教 (40793287)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 若返り / Parabiosis

Outline of Final Research Achievements

Using the skills of a surgeon with supermicrosurgery, we created and evaluated a model of parabiosis in young and old mice. Although it was difficult to create a model using vascular anastomosis, we evaluated whether a younger factor would provide a stronger rejuvenating factor to old mice by comparing young 1-month-old mice with 2-month-old mice.
The rejuvenation index, called cellular senescence-associated secretory traits (SASPs), was the target of evaluation. However, when differences in SASP factors in brain, liver, and kidney were compared, each factor did not produce a large difference. In other words, the hypothesis that younger factors have stronger rejuvenation effects may be rejected.

Free Research Field

リンパ浮腫、抗老化

Academic Significance and Societal Importance of the Research Achievements

老化研究を行う際に老齢マウスと若齢マウスを接合し、血流を交差させることで若齢マウスの血液が老齢マウスに与える影響を評価するHeterochronic parabiosisという実験手法が存在するが、本手法の若齢マウスは2ヶ月齢までの報告しかなく、また若齢マウスの月齢にはばらつきがあるため、統一された研究モデルではなかった。我々の研究では、1か月と2か月のマウスには有意差はなく、若齢側のマウスがより若くある必要がない可能性が示唆されており、老化研究者にとって重要な情報の一つとなりうる。