Study of the Promotive Effect of Low-Cytotoxic Surfactants on Cell Growth

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K18436
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Kobe University
• Principal Investigator: Ishida Yasuhisa 神戸大学, 医学研究科, 医学研究員 (10457062)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 両親媒性界面活性剤 / 線維芽細胞 / 細胞増生 / 創傷治療

Outline of Final Research Achievements

Applicants have demonstrated that the addition of an amphiphilic surfactant, Pluronic F 68, to cell culture media promotes cell growth and does not cause cytotoxicity even at high concentrations. We also compared cultured human fibroblasts with basic fibroblast growth factor alone and those with basic fibroblast growth factor and Pluronic F 68, and confirmed that cell growth was enhanced in the group with Pluronic F 68.
To clarify this principle, we added bFGF alone and bFGF + Pluronic F 68 to fibroblasts, cultured them, and measured the amounts of pERK and ERK by electrophoresis to determine the degree of activation of cell proliferation, but no significant findings were obtained.

Free Research Field

形成外科

Academic Significance and Societal Importance of the Research Achievements

生体にとって低毒性である両親媒性界面活性剤が細胞増生を促進することを明らかにすることで、今後、この両親媒性界面活性剤が創傷治療、特に難治性潰瘍の保存的治療（創傷被覆材や軟膏、洗浄剤）に活用することが出来る可能性があることを示唆している。また基礎的実験において細胞培養を行う際の添加材料として活用することが出来る可能性もあると考えた。