Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18538
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Kyushu University
• Principal Investigator: Yoshida Shinichiro 九州大学, 大学病院, 助教 (30778866)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 4-META/MMA-TBB resin / Nano hydroxyapatite / dental pulp stem cells / reparative dentin / direct pulp capping

Outline of Final Research Achievements

This study was to evaluate the dentin-adhesive 4-META/MMA-TBB resin including osteo-conductive nano hydroxyapatite as a novel direct pulp capping material. The 30% naHAp/SB showed a good sealing ability and biocompatibility as SB does, and also induced odontoblastic differentiation of human dental pulp stem cells through a CaSR-MEK/ERK pathway. In addition, 30% naHAp/SB sealed the pulp exposure site by inducing reparative dentin with dentin tubules in a rat artificail pulp exposure model. These results indicate that 30% naHAp/SB can be used as a novel direct pulp capping material.

Free Research Field

保存修復

Academic Significance and Societal Importance of the Research Achievements

これまでに歯質接着性を有した直接覆髄材は存在せず、辺縁漏洩による術後感染のリスクが存在する。本研究で開発した新規直接覆髄材は歯質接着性を有しており、辺縁漏洩のリスクを抑え長期的な予後を見込むことができる。また、材料の諸性質の解析に加えて、修復象牙質形成の主役となる歯髄幹細胞の象牙芽細胞様分化に及ぼす影響ならびにその細胞内シグナル経路の解明を行った。さらに、動物実験モデルでは良好な修復象牙質形成効果を認めており、今後臨床応用へと繋げていくことができると考えている。