Effect of low-intensity pulsed ultrasound for periodontal treatment

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K18581
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Nihon University
• Principal Investigator: MANAKA Soichiro 日本大学, 歯学部, 助教 (00754954)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 低出力超音波: LIPUS / 歯周病 / LPS

Outline of Final Research Achievements

LIPUS significantly increases gene and protein expression of multiple extracellular matrix proteins in bone tissue. We also suggested that LIPUS might affect osteogenesis through ATP-related P2X7 receptors. Assuming periodontal disease-affected alveolar bone, the effects of LIPUS, in which LPS derived from periodontal pathogenic bacteria was added to osteoblasts, were suppressed the gene and protein of inflammatory cytokines IL-1α, IL-6, and RANKL. We also suggested that LIPUS might reduce LPS-induced nuclear translocation and suppress cytokines through AT1-PLCβ in osteoblasts.
Therefore, LIPUS may calm inflammation in the affected area of periodontal disease and suppress bone resorption.

Free Research Field

歯周病学

Academic Significance and Societal Importance of the Research Achievements

歯周病を想定し、骨芽細胞に誘導したLPSおよびLIPUSにおける炎症性サイトカインの影響を検討した論文や研究発表は国内外に問わず見当たらない。そのため、世界レベルで増加傾向にある歯周病の炎症を軽減させるのに、保険適応が認められているLIPUSの影響を検討する本研究は、歯科および医科の両領域においてのLIPUS使用についてEBMの確立の一端を担うと考えられる。さらに、骨折・炎症性骨疾患により惹起された骨吸収およびインプラント治療後の骨結合（オッセオインテグレーション: osseointegration）の確保など、他分野におけるLIPUSの使用の可能性拡大が期待され、大きな意義があると言える。