2020 Fiscal Year Research-status Report
Applying the anti-inflammatory phenotypes of macrophages to reverse salivary gland inflammation in mice with abnormal phosphatase SHP2 expression
| Project/Area Number |
20K18728
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | Sjogren's syndrome / Cell therapy / Xerostomia |
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| Outline of Annual Research Achievements |
As we aim to study if E-MNC transplantation can reduce inflammation in salivary glands of Sjogren's syndrome mouse models, such as the MRL/lpr mice. Firstly, we have examined the potential of the anti-inflammatory effect of E-MNC transplantation in a well-established model of Sjogren's disease, the non-obese diabetic (NOD) mouse. Our recent data shows that after 1, 3 and 7 days of E-MNC transplantation in salivary glands of 8-week-old NOD mice, expression of inflammatory genes such as TNF-alpha, IL-1beta and interferon gamma were lower compared to untreated controls.
Moreover, through histochemical analyses, including tracking of cells through PKH-labelling, we have confirmed the effectiveness of the transplantation in the tissues after 1, 3 and 7 days. Moreover, we have confirmed the characteristics of these cells in the tissue, which preserve their phenotypes even after 3 days of transplantation.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Before proceeding with the use of our target mouse model, the MRL/lpr mouse, we have decided to analyze the anti-inflammatory effect of EMNCs, firstly, in the NOD mouse model since we have more experienced with it so we can continue and apply this experimental methods with the MRL/Lpr mouse.
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| Strategy for Future Research Activity |
Once the most effective timepoints for cellular transplantation of EMNCs have been clearly defined using the NOD mouse model, we will apply the same strategy using the MRL/Lpr mouse.
As a counter measure, if inflammation is not suppressed in the salivary glands of the MRL/Lpr mouse, it is also possible to focus on solely in the NOD mouse, including the SHP-2 expression
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| Causes of Carryover |
Fewer consumables were purchased; the materials/reagents for the initial analyses done have lower costs than the analyses planned for the next stage of the study. No travel expenses were incurred; No expenses for presentation or registration to scientific meetings were incurred. In the next fiscal year, we plan to use this amount for publication fees for the acceptance of a manuscript in a peer-reviewed journal, we plan to use this amount.
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