2023 Fiscal Year Final Research Report
Elucidate the mechanism by which lactate inhibits muscle performance decline.
| Project/Area Number |
20K19567
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 59020:Sports sciences-related
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
Tanaka Yoshinori 電気通信大学, 大学院情報理工学研究科, 協力研究員 (40791249)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | in vivoイメージング / 骨格筋 / ミトコンドリア / 乳酸塩 / カルシウムイオン |
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| Outline of Final Research Achievements |
In recent years, lactate, which is produced during metabolic activity in skeletal muscle, has been considered to contribute to the maintenance of muscle performance rather than contributing to fatigue. In this study, a model was developed to visualise changes in lactate concentration during exercise using lactate-sensitive fluorescent proteins. The results demonstrated that lactate concentration dynamics differed between muscle fibre types during high-intensity muscle contraction. Furthermore, we examined the role of mitochondria in skeletal muscle during muscle contraction. Mitochondria play an essential role in lactate metabolism, and our findings suggest that changes in mitochondrial Ca2+ concentration during contraction directly influence muscle tension level.
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| Free Research Field |
筋生理学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において確立されたバイオイメージング手法は，ホメオスタシスを維持して細胞内イオン動態の観察を可能とした．このモデルを用いて筋収縮活動時の乳酸動態とミトコンドリアCa2+動態の観察に成功した．乳酸とCa2+の細胞内での振る舞いは筋収縮の制御機構の中心であり，筋疲労の機構を解明するために特に重要である．また，本研究の研究モデルや知見は，エイジングならびに糖尿病などの代謝性疾患に付随する筋機能低下の機構解明などにも応用が期待される．
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