Identification of novel compound for alleviating skeletal muscle fatigue focusing on S-glutathionylation

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K19570
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59020:Sports sciences-related
• Research Institution: Hiroshima University
• Principal Investigator: Watanabe Daiki 広島大学, 人間社会科学研究科(総), 助教 (30823281)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 骨格筋疲労 / S-gluathionylation / スキンドファイバー / 活性酸素種 / 筋小胞体 / 筋原線維

Outline of Final Research Achievements

It has been recently shown that S-glutathionylation of troponin I ameliorates skeletal muscle fatigue. The purpose of the present study was to identify a novel compound(s) for an enhancement of glutathione-S-transferase which is a catalyst for S-glutathionylation. Present study indicated that indole-3-carbinol significantly decreased GST activity and suppression of S-glutathionylation delayed recovery from muscle fatigue. Although the enhancer of GST activity was not identified in this study, this study may provide a clue to develop the new compound alleviating muscle fatigue.

Free Research Field

筋生理学

Academic Significance and Societal Importance of the Research Achievements

本研究では，グルタチオン（GSH）化による骨格筋疲労の抑制効果を高める化合物の開発を試みた．結果的には，そのような化合物の発見には至らなかったが，GSHの筋疲労に対する役割を詳細に検討できた．本研究では，GSHは，1) 筋疲労を迅速に回復させる役割があること，2) 疲労発生初期においては，GSHには正の効果だけでなく負の効果もあることを示した．これらの結果は，GSHを用いた治療の有用性を示すとともに，その負の面も示しており，今後の筋疲労の治療法に対して新たな基盤を提供したものと考えられる．