2023 Fiscal Year Research-status Report
Development of cell-based stress evaluation assays for drug/compounds screening.
| Project/Area Number |
20K19700
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Saiki Papawee 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (10803934)
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| Project Period (FY) |
2020-04-01 – 2025-03-31
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| Keywords | stress / immune system / microflora |
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| Outline of Annual Research Achievements |
I have moved to management office for 1 year (2023/5/1-2024/4/30). I do not have much time to complete this project. So I extended this project to finish in this year. However, I found that IL-6 and IL1b gene expression in Payer's patch was significantly increased in stress mice. Payer`s patch is a part of intestine that it may be a key important of relationship of immune system and microflora. I also had a poster presentation in 日本睡眠学会第45回定期学術集会・第30回日本時間生物学会学術大会 合同大会 on September 16th, 2023 for investigating the microbiota-brain-gut axis via the immune system in a mouse model of chronic stress.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
I took maternity leave in 2022 and then moved to the management office for 1 year (2023/5/1-2024/4/30). I could not do research during maternity leave and have not much time to complete research. So the research plan was delayed. But I plan to complete this research within this year.
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| Strategy for Future Research Activity |
I plan to publish a publication this year. Based on results, I will develop cell-based assay for suppression of IL-6 by induction of TNFa or Il1b in payer`s patch or intestine cell. I will ty to do three kinds of cell types as follows.
1) primary cell from payer`s patch of mice
2) Caco2 cells
3) Caco2 coculture with Raw264.7
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| Causes of Carryover |
I will use grant for develop cell based-assay in this year (purchase cell line, medium, ELISA kit, qPCR kit for cytokines expression) etc. I will use grant for publication and conference.
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