2022 Fiscal Year Annual Research Report
The establishment of a biomimetic human iPSC-derived cornea-on-a-chip for the pre-clinical evaluation of ophthalmic nanomedicines
| Project/Area Number |
20K20168
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
ABDALKADER Rodi 立命館大学, 立命館グローバル・イノベーション研究機構, 助教 (20839964)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | hPSCs / Cornea-on-a-chip / Microfluidic / Nano toxicity |
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| Outline of Annual Research Achievements |
In this project, a human corneal epithelium-on-a-chip (CEpOC) was created to test the efficacy and toxicity of ophthalmic drugs. The CEpOC was generated in microfluidic devices under eye-blinking-like stimuli. Moreover, metabolite transportation and secretion in CEpOC were assessed by untargeted metabolomic analysis, revealing biologically relevant metabolite changes, where a group of metabolites (e.g., glutathione and uric acid) were annotated as potential markers for cell toxicity. Furthermore, a simplified small molecule-based corneal induction method (SSM-CI) was developed to generate functional corneal epithelial cells from human pluripotent stem cells (hPSCs). This method resulted in mature corneal epithelial cells that have the potential to be used in CEpOC.
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