2022 Fiscal Year Annual Research Report
A mechanical study of lumen formation through membrane bleb regulation under hemodynamic influence
| Project/Area Number |
20K20190
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
MaungYe SweSoe 国立研究開発法人理化学研究所, 生命機能科学研究センター, 基礎科学特別研究員 (60866408)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Microhemodynamics / Hematocrit asymmetry / Wall shear stress / Vascular morphogenesis / Membrane blebbing / RBC mechanics / Endothelial mechanics / Vascular mechanics |
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| Outline of Annual Research Achievements |
1. A semi-automated blood flow imaging method was established for high throughput analysis of growth and hemodynamic trends in zebrafish populations. This work provides the overarching spatiotemporal systemic hemodynamic trends and vascular network level adaptations in response to local lumen morphogenesis.
2. We have established a methodology pipeline to predict high resolution distribution of wall shear stress (WSS) and blood pressure in a zebrafish trunk lumen network at 54 hours post-fertilization (hpf). 3D model of the vessel lumen network in silico was constructed from confocal imaging of the fluorescently labelled blood lumen through microangiography. Blood flow map pertaining to the vessel lumen network was reconstructed by our in-house developed CFD code after iterating model boundary conditions to match blood flow velocity data obtained from particle tracking velocimetry of red blood cells (RBCs) under high-speed camera imaging.
3. Hemorheological and lumen size alteration effects on network blood flow and forces: We studied with the CFD, network flow adaptations in response to blood viscosity and RBC concentration changes, namely how network pressure changes under network constraints of blood flow maintenance. The hemodynamic constraints applied in the model were obtained from experimental observations of network flow parameters between wild-type versus altered networks. Additionally, we also showed how different network optimizations can lead to diametrically opposite effects on WSS distribution after lumen size reduction is applied to a network.
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