2021 Fiscal Year Research-status Report
Collagen microfibers size effect on the reconstruction of vascularized adipose tissues for soft tissues regeneration applications
| Project/Area Number |
20K20199
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| Research Institution | Osaka University |
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Principal Investigator |
Louis Fiona 大阪大学, 工学研究科, 特任助教(常勤) (70838523)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | adipose tissue / vascularization / autologous / bioreactor / bioprinting / injection |
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| Outline of Annual Research Achievements |
First, the use of only autologous cells for the construction of the adipose ball tissues was confirmed by performing the endothelial differentiation from the adipose-derived stem cells (yield rate was 33%). The upgraded vascularized adipose balls also showed good tissue maintenance and vascularization after 2 weeks of implantation.
Next, the scale-up of the process was done by using a bioprinter for the seeding of the tissues and then a bioreactor for the culture (up to 100 tissues together was confirmed).
In parallel, one of our publication on this model required additional experiments during the reviewing process and we thus performed a RNA sequencing to further prove the highest tissue survival of our model compared to the controls.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The differentiation into endothelial cells using the adipose stem cells gave promising results which will were useful for the next step concerning clinical applications. As we need a mixture of adipose endothelial cells and adipose stem cells, it was not necessary to improve more the percentage of around 30% obtained for endothelial cells differentiation, which was already suitable.
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| Strategy for Future Research Activity |
The future work will focus on the confirmation of the use of only autologous cells for the construction of the adipose ball tissues. Bigger size animals will be used, for instance rabbits as well, to also validate the scale-up process. For this purpose, a bigger size bioreactor was ordered to culture 1000 tissues together.
Also, the cell culture medium will be improved to be fully suitable for clinical applications.
In parallel, the remaining publication on this model which is currently under review will be probably finally published and another one will be considered with the results of the full autologous model.
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| Causes of Carryover |
Travelling for international conferences was still prohibited so the budget planned for plane tickets and hotels was not used.
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