2021 Fiscal Year Annual Research Report
Overcoming multi drug resistance in 3D breast cancer cell model by pH-sensitive biomimetic nanoparticles
| Project/Area Number |
20K20203
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| Research Institution | Kumamoto University |
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Principal Investigator |
Lee Ruda 熊本大学, 産業ナノマテリアル研究所, 准教授 (00802050)
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | Multidrug resistance / Biomimetic nanoparticles / cell membrane / Breast cancer |
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| Outline of Annual Research Achievements |
The biomimetic nanoparticles were modified with a red blood cell membrane. Western blot, TEM, size, and zeta potential confirmed the ideal nanoparticle versus membrane ratio. The results demonstrated that successfully developed red blood cell membrane-coated nanoparticles.
For the 3D cell culture system, fibroblast, monocyte/neutrophil cells, and tumor cells were transfection using the pCMV vector and building the most similar tumor microenvironment. Currently, we evaluate the immune system escape abilities of red blood cell membrane-coated biomimetic nanoparticles.
Furthermore, I tried the research to enhance immune escape and targetability using peptide ligand. As I expected, peptide modification increased the cellular internalization of membrane-coated biomimetic nanoparticles.
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