Synthesis and mechanistic elucidation of homo poly-gamma-L- and -D- glutamic acids

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K20371
• Project/Area Number (Other): 18H05360 (2018-2019)
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund (2020); Single-year Grants (2018-2019)
• Review Section: Medium-sized Section 38:Agricultural chemistry and related fields
• Research Institution: Kobe University
• Principal Investigator: Ishikawa Shu 神戸大学, 科学技術イノベーション研究科, 准教授 (30359872)
• Co-Investigator(Kenkyū-buntansha): 戸谷 吉博 大阪大学, 大学院情報科学研究科, 准教授 (70582162)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Keywords: γ-L-PGA / 枯草菌 / オーバーフロー代謝 / 繊維状化細胞

Outline of Final Research Achievements

The molecular mechanism of the synthesis of DL-PGA and L-PGA was elucidated, and a mutant strain with higher productivity was successfully obtained than wild-type PgsA. We also succeeded in creating a strain deficient in the acetoin-acetate synthesis pathway without growth inhibition, which leads to improved PGA productivity, and in developing fibroblasts. We established a screening method for strains with enhanced D-PGA synthesis, but we could not obtain a D-PGA-producing mutant strain. However, we developed a new method to obtain mutant strains of D-PGA synthesis.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は信頼性の高いPGA合成の分子メカニズムのモデル提唱に至り、その学術的意義は計り知れない。特に、L-PGA高生産変異株の取得、超高分子L-PGA生産技術開発は画期的である。さらに新規代謝システム構築と繊維状細胞構築の成功は、工業スケールの生物生産プロセス改良へと繋がる。これらの成果は社会全体に影響を及ぼす可能性がある。