2022 Fiscal Year Final Research Report
Reconstitution of artificial blastocysts by stem cells
| Project/Area Number |
20K20375
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| Project/Area Number (Other) |
18H05366 (2018-2019)
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| Research Category |
Grant-in-Aid for Challenging Research (Pioneering)
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| Allocation Type | Multi-year Fund (2020)
Single-year Grants (2018-2019) |
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| Review Section |
Medium-sized Section 42:Veterinary medical science, animal science, and related fields
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| Research Institution | Chiba University (2018, 2020-2022)
Institute of Physical and Chemical Research (2019) |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2023-03-31
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| Keywords | 原始内胚葉 / 胚盤胞 / 胚オルガノイド |
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| Outline of Final Research Achievements |
The blastocyst is composed of three cell lineages: preimplantation epiblast, trophoblast, and primitive endoderm. ES cells have been established from the preimplantation epiblast and TS cells from the trophoblast, but stem cells from the remaining primitive endoderm have not yet been established. We report the successful establishment of primitive endoderm stem cells in serum-free medium supplemented with FGF4, CHIR99021, and PDGF-AA. After all the cell lineage compornents necessary for the production of artificial blastocysts were prepared, we next produced early embryo organoids with ES cells, TS cells, and PrES cells, and transferred them into the uteri of pseudopregnant mice. These artificial embryos implanted efficiently and derived embryo-like structures surrounded by visceral yolk sac and parietal yolk sac-like structures.
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| Free Research Field |
発生生物学
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| Academic Significance and Societal Importance of the Research Achievements |
胚盤胞を構成する3つの細胞系譜の内、最後に残された原始内胚葉系列からPrES細胞が樹立されたことにより、概念的にはES細胞、TS細胞、PrES細胞を組み合わせて用いることで、胚盤胞を再構成できる可能性が示唆された。幹細胞のみで胚を作製する研究は、近年、幹細胞研究分野の中でも最も競争の激しいテーマとなったが、PrES細胞の樹立により、分野の発展はさらに加速すると予想される。
生命発生現象を幹細胞間相互作用として捉え、理論を解明する技術的基盤が確立されたことにより、生命とは何かという人類の根源的問いを解き明かす糸口を作ることができた。
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