Development of Diodegradable Silk Protein Scaffolding Material and Application to Cell Culture Plates by 3D Printing

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K20430
• Project/Area Number (Other): 19H05504 (2019)
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund (2020); Single-year Grants (2019)
• Review Section: Medium-sized Section 18:Mechanics of materials, production engineering, design engineering, and related fields
• Research Institution: Tohoku University
• Principal Investigator: Narita Fumio 東北大学, 環境科学研究科, 教授 (10312604)
• Co-Investigator(Kenkyū-buntansha): 栗田 大樹 東北大学, 環境科学研究科, 助教 (40643226)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: 材料力学 / 3D印刷 / 複合材料 / 生体材料 / 人工臓器

Outline of Final Research Achievements

First, this study showed that the enhanced mechanical properties of the cellulose nanofiber (CNF)/silk fibers are attributed to the improved alignment and enhanced dispersion of CNFs inside the silk fiber. Then, PEGDMA/silk fibroin (SF) hydrogels with varying concentrations of SF were developed by digital light projector. The elastic compressive modulus of the printed hydrogel had a wide range of values, suggesting that it could be a good substitute for various stem cells from cartilage, muscle tissue, fat, and bone.

Free Research Field

複合材料設計学

Academic Significance and Societal Importance of the Research Achievements

近年，工業分野では3D印刷技術が著しく発達し，大きな注目を集めている．本研究では，シルクタンパクの3D印刷を実施し，PEGDMA/シルクフィブロインハイドロゲルの光造形に成功して，ハイドロゲルが生体足場材料として使用できる可能性を示した．今後発展が予想されるヒトの組織を造形する3Dバイオプリンティング技術において，本研究から得られる基礎的知見は極めて有用である．