Non-intrusive measurement of intercellular ions distribution by total internal reflection mapping method between vibration modes and hydrogen bonding of water molecules

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K20431
• Project/Area Number (Other): 19H05505 (2019)
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund (2020); Single-year Grants (2019)
• Review Section: Medium-sized Section 19:Fluid engineering, thermal engineering, and related fields
• Research Institution: Keio University
• Principal Investigator: SATO Yohei 慶應義塾大学, 理工学部(矢上), 教授 (00344127)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 全反射ラマン分光法 / 全反射ラマン散乱光イメージング法 / ラマンスペクトル解析法 / 水分子動態 / 水素結合状態 / イオン動態 / イオン周りの水和殻状態 / 細胞内液

Outline of Final Research Achievements

Dynamics of water molecules and ions in electrolyte solutions were investigated by development of total internal reflection (TIR) Raman microscopy. In this study, TIR Raman spectroscopy and TIR Raman imaging were proposed, whose signals have been considered as the low signals-to-noise ratio. The new type of analysis methods using the Raman spectra and the Raman images from water molecules were proposed to investigate the dynamics of the hydrogen bonding of water molecules and the hydrogen shell around ions. The obtained results will provide further insight into the dynamics of intra-and intercellular water molecules.

Free Research Field

熱流体工学

Academic Significance and Societal Importance of the Research Achievements

水分子およびイオンの動態は、現在まで古典物理や古典化学に基づいて議論されてきたが、イオン介在による物理現象を的確に把握することが困難であった。本研究では実験的に初めて、イオン周りの水分子の水素結合状態、そして水和殻状態を定量的に把握し、様々な物理パラメータとの関連性を明らかにした点は、学術的意義を有している。細胞内液には様々なイオンが存在していることから、イオンが病に及ぼす影響の解明に貢献できると期待できる。