2022 Fiscal Year Final Research Report
Function of non-coding RNA in the development of fatty liver injury induced by a high-calorie diet
Project/Area Number |
20K20479
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Project/Area Number (Other) |
19H05572 (2019)
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Research Category |
Grant-in-Aid for Challenging Research (Pioneering)
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Allocation Type | Multi-year Fund (2020) Single-year Grants (2019) |
Review Section |
Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
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Research Institution | Nagasaki University |
Principal Investigator |
MORI Ryoichi 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (30509310)
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Project Period (FY) |
2019-06-28 – 2023-03-31
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Keywords | microRNA / 老化 / 寿命 / 脂肪肝 / ミトコンドリア / 代謝 |
Outline of Final Research Achievements |
The microRNA miR-142 is involved in lipid metabolism and is associated with aging. The dysfunction of miR-142 has been suggested to affect lifespan, but the molecular mechanism is unknown. We examined mitochondria derived from miR-142 knockout (KO) mouse liver using a focused ion beam/scanning electron microscope. We found that the KO mice had morphological abnormalities. In addition to these morphological abnormalities, the miR-142 KO liver-derived mitochondria showed an increase in beta-oxidation (hyperactivation of lipid metabolism). As a result, the development of age-related fatty liver was suppressed in miR-142 KO mice. These findings suggest that the cause of the shortened lifespan in miR-142 KO mice is mild chronic mitochondrial dysfunction in the liver.
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Free Research Field |
実験病理学
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Academic Significance and Societal Importance of the Research Achievements |
ミトコンドリア機能異常は様々な病態発症の原因となるため、長らく注目されている細胞内小器官である。本研究は、非コード RAN である miR-142 がミトコンドリア機能に関与し、さらに寿命に影響を与えていることを見いだした。また、生体における miRNA とミトコンドリア機能との関係、さらにその機能破綻による生体へ影響を実験病理学的に示した。今後は、miR-142 を標的とした加齢性脂肪肝発症抑制に資する薬剤の開発を進捗させて、新規治療法開発へと繋げたい。
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