2023 Fiscal Year Final Research Report

Study on the mechanism of neuron cell activity induction and motion induction by ultrasound stimulation

Research Project

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Project/Area Number	20K20642
Research Category	Grant-in-Aid for Challenging Research (Pioneering)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 90:Biomedical engineering and related fields
Research Institution	The University of Tokyo
Principal Investigator	Takagi Shu 東京大学, 大学院工学系研究科(工学部), 教授 (30272371)
Co-Investigator(Kenkyū-buntansha)	関和彦国立研究開発法人国立精神・神経医療研究センター, 神経研究所モデル動物開発研究部, 部長 (00226630) 神保泰彦東京大学, 大学院工学系研究科(工学部), 教授 (20372401) 榛葉健太東京大学, 大学院工学系研究科(工学部), 助教 (80792655)
Project Period (FY)	2020-07-30 – 2024-03-31
Keywords	ニューロモジュレーション / 神経細胞 / 超音波 / カルシウムイオン / マウス / 発火 / 運動誘発
Outline of Final Research Achievements	In the present study, we obtained information on calcium release from neuronal cell populations cultured on a substrate by ultrasound irradiation. In particular, we observed ultrasound response characteristics in a group of neurons with different number densities and confirmed that the ultrasound response is a response involving synaptic transmission. Furthermore, under conditions in which excitatory synaptic transmission was inhibited, activity was induced in only some neurons, indicating that synaptic transmission is not required for ultrasound response. Furthermore, by using microbubbles, the force acting on neuron cells is localized to cell scales. This gives the calcium ion releasing with lower amplitude of irradiated pressure.
Free Research Field	生体力学
Academic Significance and Societal Importance of the Research Achievements	本研究で得られる知見は，脳神経系への超音波照射により運動を誘発する超音波ニューロモジュレーションへと繋がる知見を与える．超音波ニューロモジュレーションは，従来の電場・磁場を用いる手法に比べ，脳深部への局所的刺激を非侵襲で行える可能性を有するものであり，リハビリテーションへの利用など臨床応用への期待される．