2022 Fiscal Year Final Research Report
Universal evaluation of biological activities for bioassay based on optical image conversion
| Project/Area Number |
20K21152
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 30:Applied physics and engineering and related fields
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ラジャゴパラン ウママヘスワリ 芝浦工業大学, 工学部, 教授 (40270706)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | Laser speckle / bioassay / micro-bioassay / biospeckle / environmental txicity |
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| Outline of Final Research Achievements |
Recently, a direct toxicity assessment (DTA) method called bioassay, which comprehensively evaluates toxicity by using organisms such as bacteria, algae, and plankton without specifying individual environmental pollutants, has attracted attention. However, the conventional method of identification using a microscope cannot process a large number of individuals due to focal depth of the imaging system.
In this study, we develop an on-site, rapid, and inexpensive method to evaluate the motor activity of these indicator microorganisms as an aggregate rather than focusing on individual organisms. This method evaluates the swimming ability of plankton under environmental toxicity based on the rapid fluctuation of biospeckle generated when plankton are illuminated by laser light. Since the method does not require conventional microscopic observation, it is possible to evaluate thousands to tens of thousands of plankton at a time.
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| Free Research Field |
光計測
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，個々の個体に注目するのではなくこれら指標微生物の集合体としての運動活性をオンサイトで迅速かつ安価に評価する光学的手法の開発をおこなった．本手法では，対象生物が菌糸やプランクトンのように全く異なる構造であっても共通したスペックルパターンへと画像変換されるので，生物試料の形態によらず解析手法は共通した方法をとることができるという利点がある．加えて，光学系が単純であるのでオンサイトで迅速かつ安価にバイオアッセイをおこなうことができる．東南アジアなどの途上国では地下水のヒ素などによる汚染のために健康被害が生じており，開発手法はこのような地域で特に有効である．
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