2021 Fiscal Year Final Research Report
Plasmonic waveguiding remote surface enhanced fluorescence microscopy
Project/Area Number |
20K21165
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 32:Physical chemistry, functional solid state chemistry, and related fields
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Research Institution | Hokkaido University |
Principal Investigator |
Hiroshi Uji-i 北海道大学, 電子科学研究所, 教授 (40519352)
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Co-Investigator(Kenkyū-buntansha) |
猪瀬 朋子 京都大学, 高等研究院, 特定助教 (10772296)
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Project Period (FY) |
2020-07-30 – 2022-03-31
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Keywords | 近接場顕微鏡 / DNAマッピング / プラズモニクス / 銀ナノワイヤー |
Outline of Final Research Achievements |
In this study, we aimed to develop a new "remote-excitation probe-enhanced fluorescence (RE-TEF) microscope" with high spatial resolution of less than 10 nm by introducing silver nanowires (AgNWs) of 100 nm in diameter and several tens of um in length, chemically synthesized to function as a plasmon waveguide, on an AFM cantilever In this study, we comparatively developed a method of galvanic substitution reaction, photo-induced local deposition of gold nanoparticles, and alignment of two AgNWs to couple far-field light in propagating plamons. These probes enable spectral mapping with a spatial resolution of about 50-100 nm, which is useful for novel optical DNA mapping analysis.
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Free Research Field |
顕微分光
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Academic Significance and Societal Importance of the Research Achievements |
蛍光遺伝子マッピングは染色体に存在する特定の遺伝子配列部位の相対位置を蛍光測定で特定することであり、ゲノム配列決定、医療診断、病原同定などに用いられる臨床でも重要な技術である。シークエンスでは数週間かかる判断が、蛍光マッピングでは数日内に行えるため、迅速な治療判断が必要な場合にその威力を発揮する。本研究で開発いたリモート励起TEFは高い空間分解能を有するため、新たなDNAマッピング技術などに応用することで医療分野への高い貢献度が期待される。
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