Cryogenic single-molecule fluorescence imaging for deep inside of cell

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K21168
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 32:Physical chemistry, functional solid state chemistry, and related fields
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Matsushita Michio 東京工業大学, 理学院, 准教授 (80260032)
• Project Period (FY): 2020-07-30 – 2022-03-31
• Keywords: １分子分光

Outline of Final Research Achievements

Recently, we have been developing the cryogenic fluorescence microscope for nanometer-level imaging (cryo-nanoscope). Although the cryo-nanoscope could be localized the small biomolecules with a nanometer precision, the field-of-view was as small as the size of cell. Here we study a light-sheet excitation for cryo-nanoscope. We examine the two types of the light-sheet excitation methods by wave optical simulation. As a result, the light-sheet excitation using a two objective lens having a NA of 0.6 was much better than that using a one objective lens.

Free Research Field

物理化学

Academic Significance and Societal Importance of the Research Achievements

本研究はナノレベルのイメージング法を細胞へ応用するために必須の技術であり、学術的な意義は大きい。また、この技術を生体組織や個体に応用していけば、生命の謎を分子レベルで解明することが可能になり、学術的にも社会的にも大きな意義を持った研究となるはずである。