Elucidation of the mechanisms by which endobacteria give symbiotic ability to Mortierella spp.

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K21301
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 39:Agricultural and environmental biology and related fields
• Research Institution: Ibaraki University
• Principal Investigator: Narisawa Kazuhiko 茨城大学, 農学部, 教授 (90431650)
• Co-Investigator(Kenkyū-buntansha): 晝間 敬 東京大学, 大学院総合文化研究科, 准教授 (20714504) ; 西澤 智康 茨城大学, 農学部, 准教授 (40722111)
• Project Period (FY): 2020-07-30 – 2022-03-31
• Keywords: 内生細菌 / Mortierella属菌 / 共生 / 植物生育促進

Outline of Final Research Achievements

We succeeded in culturing Mycoavidus sp. S2-EB, which is the BRE of Mortierella humilis S2. Mycoavidus sp. S2-EB was found to carry 2,575,677 bp chromosomes and 99,085 bp plasmids.
Confrontation culture of M. verticillata YTM181BF1 + Mycoavidus sp. S2-EB was performed on LCA medium. Originally, the BRE endogenous to M. verticillata YTM181 belongs to Group C, but what was detected after the confrontation culture was the BRE belonging to Group A used in the reintroduction method.
This result indicates the possibility of successful reintroduction of BRE into Mortierella spp.

Free Research Field

農学

Academic Significance and Societal Importance of the Research Achievements

本研究では、菌類-内生細菌を共生系としてとらえ、内生細菌を操る手法の開発を目的としている。この技術は、菌類の腐生性を共生に変え作物生産へ利用することへつながる。我々が菌類として認識していた生物が、いわば菌類と細菌の共生体であるという、これまでの菌類学の概念を覆し、新たな生物共生系の存在を示すことが可能となる。