2022 Fiscal Year Final Research Report
C3-C4 cytoplasmic replacement via production of hybrid zygotes and regulation of genome dymamics
| Project/Area Number |
20K21317
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
|
|---|
| Research Institution | Tokyo Metropolitan University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
石井 孝佳 鳥取大学, 乾燥地研究センター, 准教授 (80823880)
|
|---|
| Project Period (FY) |
2020-07-30 – 2023-03-31
|
| Keywords | 交雑受精卵 / 細胞質置換 / 細胞質雑種 / C4型光合成 / C3型光合成 / コムギ / トウモロコシ / パールミレット |
|---|
| Outline of Final Research Achievements |
Hybridization drives speciation and generates biodiversity, and wide hybridization plays a pivotal role in enhancing and broadening the useful attributes of crops by crossing species from distant gene pools. In the project, gametes isolated from C4-photosynthetic crops, such as maize and pearlmillet, and wheat, an C3-photosynthetic crop, were used to generate allopolyploid zygotes in order to generate wheat plants possessing C3 and C4 cytoplasm. Allopolyploid wheat-maize hybrid zygotes with various gamete combinations have been produced and developed into possible wheat-maize hybrid plants, which appeared to possess a wheat nuclear genome and a wheat + maize cytoplasmic genome. In addition, allopolyploid wheat-pearlmillet hybrid zygotes with various gamete combinations were also produced and developed into possible wheat-pearlmillet hybrid plants, which appeared to possess a wheat nuclear genome and a wheat + pearlmillet cytoplasmic genome.
|
| Free Research Field |
植物発生学
|
| Academic Significance and Societal Importance of the Research Achievements |
核と細胞内小器官（細胞質）との間には密接な情報と物質のやり取りがあり、これらの相互作用の上に真核細胞の存在がある。本研究の核心は、異種の配偶子を融合させることで異なる種の核と細胞内小器官を一つの細胞に共存させ、異種間の核－細胞内小器官の関係性を浮かび上がらせる点にある。本研究では、新たな核－細胞内小器官の組み合わせをもつ細胞の作出に成功し、異種間の核－細胞質の相互作用解析への礎を築いた。また、「核ゲノムと細胞内小器官（細胞質）の人為的組み合わせによる植物の新形質」という観点から考えた際には、「C3型光合成を行うコムギ細胞質のC4型光合成への置換」に向けた大きな一歩といえる。
|
