2022 Fiscal Year Final Research Report
Development of an environmental RNA method for estimating growth stages of fish: Japanese eel (Anguilla japonica) as a model
| Project/Area Number |
20K21335
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
土居 秀幸 兵庫県立大学, シミュレーション学研究科, 准教授 (80608505)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | 環境RNA / 環境mRNA / ニホンウナギ / 成育段階 / 環境DNA / 銀ウナギ / 黄ウナギ / RNA-seq |
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| Outline of Final Research Achievements |
In this study, we attempted to develop an environmental RNA (eRNA) method that can distinguish and quantify different growth stages using the Japanese eel, an important fishery resource, as a model case. As a result of our field survey at Lake Jinzai in Shimane, it was found that eRNA of this species is extremely difficult to detect compared to eDNA, and that the digital PCR method may be effective in detecting eRNA. We also showed that benzalkonium chloride, which has an inhibitory effect on eDNA degradation, is also effective against eRNA. In addition, we succeeded in developing a primer/probe for one of the gonadotropins of this species. The RNA-seq analysis using fin samples of this species suggested the presence of some genes that are stage-specifically expressed. In the future, we plan to examine the points that need to be improved, which were clarified in this study, toward the establishment of an eRNA method.
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| Free Research Field |
陸水生態学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果として、これまで未解明であった環境RNAの自然環境中の動態の一端を解明し、環境RNA手法の実用化に向けた基礎的な知見を収集できたと考えている。これらの成果をベースにして環境RNA手法を確立できれば、野外では水サンプルを採取するだけの簡便な操作のみによって、とくに産卵に向かう銀ウナギ（＝下りウナギ）を科学的根拠に基づいて明瞭に識別・定量することが可能になり、効果的な漁獲制限によって将来的な資源回復への貢献が期待できる。今後は、環境RNA手法の確立に向けて、環境中のRNAの回収率を高めるための採水・濾過量の増加やデジタルPCRの費用対効果などの検討を進めていく必要があると考えている。
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